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If I play Guilty or Innocent on Rippersnapper and choose the "Enemies can't use Tough this battle" option, does that mean Rippersnapper's bot mode will reduce the defender's defense?

No. Guilty or Innocent simply prevents the defender from using Tough. It does not get rid of it. Therefore, the defender is still a defender with Tough, and Rippersnapper cannot use his bot mode ability as a result.
Source: Aequitas (2023-07-05)    【Link to Ruling】
Tags: Guilty or Innocent, Terrorcon Rippersnapper, Tooth and Nail