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Can I play this card if only one or two of the characters are in the KO area?

Yes. You’ll put as many of the three characters onto the battlefield as you can. For example, let’s say you have Full-Tilt on the battlefield, Wipe-Out in the KO area, and Brunt under Trypticon. Playing Relentless Invasion will put Wipe-Out onto the battlefield. It will attack this turn and then be put back under Trypticon. Full-Tilt doesn’t have to attack, and at the end of the turn it will stay on the battlefield.
Source: Wizards of the Coast (2019-11-08)    【Link to Ruling】
Tags: Relentless Invasion