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Autobots have a red frame and the Autobot insignia in the lower right corner. Decepticons have a purple frame and the Decepticon insignia in the lower right corner. A few characters have "Autobot" or "Decepticon" in their names, although these arent the only Autobots and Decepticons.
Yes. The faction symbols as well as the different frame/treatment at the top and above the Battle Card text for each faction.
No. In order to qualify as "only Autobots," your starting deck can't have any characters that are another faction (even if they're also Autobots).
Different faction means "does not share a faction identity." For a character which is both an Autobot and a Decepticon, "not sharing a faction identity" means NOT Autobot and NOT Decepticon. Currently, this leaves Mercenary as the only different faction.
Characters like Optimus Prime, Dark Awakening belong to multiple factions at once - Autobot and Quintesson. Characters with multiple factions will not allow for card effects that require only one faction to be on your starting team to be used. However, characters with more than one faction may qualify to use certain upgrades that require one of those factions.
For example, if Optimus Prime, Dark Awakening (Autobot/Quintesson) is on your starting team, you cannot use Heroic Spotlight to include 2 ★ worth of blue cards in your deck.
For battle cards like Confidence that requires your starting team to be only Autobots would not work in this example. You could draw 2 cards, then scrap 2 cards, but you would not get to play another action.
Additionally, both Armada, Herald of Shrouds (Decepticon/Unicronian) and Decepticon Exhaust (Decepticon/Mercenary) would be able to use a Scoundrels Blaster.